Advancements in Clinical and Medical Research

L actic acid bacteria (LAB) were isolated from cowmilk yoghurt by culturing the milk on specific media and pure culture was obtained by sub-culturing. Preliminary identification of lactic acid bacteria was made on the basis of Gram-staining and catalase reaction followed by microscopic examination to observe cell arrangement and morphological characteristics. After incubation, individual colonies were selected and moved into sterile brooths (MRS or M17 medium for enrichment). The enriched bacteria were purified using streak plate technique. Gram positive and catalase negative cocci and bacilli colonies were stored in glycerol solution as lactic acid bacteria. Cowmilk yoghurt contained lactic acid producing bacteria, four bacterial strains were isolated, according to biochemical identification and were characterize as: Lactobacillus rhaminosus-2, Lactobacillus fermentum, Lactobacillus paracasei-2, and Lactobacillus licheniformis-2. The isolates were characterized on the basis of t...

M onkeypox is a neglected tropical zoonotic disease endemic in the tropical rainforest regions of Central and West Africa. The virus causing it, the Monkeypoxvirus (MPXV), is so-called because it was first found in   Cynomolgus monkeys , in Denmark, back in 1958. However, primates, including humans, are now suspected to be only incidental hosts, though there is little evidence to support the hypothesis that small mammals, including rodents, are the natural hosts. Since the first human case of monkeypox in 1970, several outbreaks have been reported, mostly in the Democratic Republic of Congo and Nigeria, with over 19,000 cases documented over the two decades from 2000-2019. Since then, almost 16,000 cases have been reported from 2021-22. This indicates the rapidly increasing scale of  infection . This could be due to many different reasons: better surveillance and reporting, cutting down of forests and thus greater contact with animal reservoirs, loss of poxvirus immunity as th...

G astrointestinal stromal tumor (GIST) constitutes up to 1% of all malignant neoplasms of the gastrointestinal tract, with the stomach and small intestine being the most frequently affected organs. The purpose of this report is to present the first published case of synchronous duodenal and jejunal GISTs. A 57-years old male patient with type II diabetes complained of asthenia and vertigo, resulting from anemia. On upper digestive endoscopy, a friable lesion was found in the second portion of the duodenum. The biopsy diagnosed GIST and its removal was indicated. During the surgical procedure, a second tumor was found in the jejunum, which was also removed. Anatomopathological examination of this second lesion also revealed GIST with the same cytological features. Digestive reconstruction was performed in Roux-en-Y of the jejunum sectioned distally to the tumor with the open part of the duodenum. The patient evolved without complications, was discharged on the third postoperative day an...

M en's lives have failed to establish balance between the body, mind, and spirit as a result of changing lifestyles, job stress, and environmental degradation. In this circumstance, Ayurveda has stepped in to save those lives by providing a calm healthcare system. Ayurveda, which is over 1000 years old, has a long history of being practiced mostly in the Indian subcontinents, with Kerala, a south Indian state, offering a variety of therapies and procedures to treat various aches, pains, and chronic ailments through Ayurveda. As a result, individuals from neighboring states, as well as foreigners, are flocking to Kerala in the hopes of being healed via a holistic treatment. The objective of the research was to find out how Indian and international healthcare customers viewed performance-based service quality in Ayurveda healthcare. A standardized questionnaire was disseminated to the 20 Ayurveda hospitals using a suitable sampling approach, and 404 domestic and 82 foreigners took ...

Researchers investigated the association between a complete 10-second one-legged stance and all-cause   mortality . Aging is correlated to the progressive decline in physical health and reduction in elements of aerobic and  non-aerobic fitness . Moreover, several studies have shown a reduction in balance after the age of 55 years, thus increasing the odds of sustaining a fatal fall and other adverse outcomes. Study Recently, Researchers assessed the correlation between a complete 10-second one-legged stance (10–second OLS) and all-cause mortality and  prognostic data . The team conducted a prospective cohort study with data collected from the CLINIMEX Exercise open cohort protocol. The study included a total of 1702 participants aged between 51 and 75 years at the time of the first evaluation, performed between 10 February 2019 and 10 December 2020. These participants had voluntarily visited the clinic to assess their aerobic and non-aerobic  physical fitness . The t...

INTRODUCTION Metabolic illnesses are currently a worldwide pandemic. Physical activity is a widely accepted method to improve metabolic health. Identifying aspects of childhood experience that positively affect a person's life-long physical activity levels is vital to reducing the effects of metabolic illness. Objective: To gain perspective of child-parent relationship qualities that correlate with positive metabolic health and physical activity levels into adulthood. METHODOLOGY 233 participants participated in a survey gathering information on self-reported physical activity, sedentary behaviour, and familial background. Five subjects then participated in interviews, building in-depth insight into the information provided in the survey. RESULTS Subjects belonging to families with a lack of perceived support for sports participation were significantly more likely to be classified as sedentary adults. Discussion: Correlations between physical activity's ubiquity align with posi...

Bittmann S, Luchter E, Moschüring-Alieva E, Bittmann L, and Villalon G Ped Mind Institute (PMI), Department of Pediatrics, Germany Since Corona Pandemic the role of telemedicine became more important. Since years, telemedicine settings were used in adult medicine and to date, in few pediatric hospitals, ambulances and institutions. In 1500 pediatric patients, we performed 120 telemedicine consultations in the last 3 months (8.6%). In 120 consultations, there were only 3 misdiagnosis and in 8 cases, the prescriptions had to be changed. In general, it is necessary that the pediatrician is well trained and has a lot of experience in pediatric medicine . We present a new diagnostic questionnaire for telemedicine in Pediatrics. In principle, the advantages outweigh the disadvantages in the use of telematics from the point of view of the medical profession, with only a minority pointing out the disadvantages in general. 73 percent of physicians emphasize that the use of telematics in heal...

INTRODUCTION Resections for pancreatic cancer have been carried out for 86 years. Doubts about the efficacy were first expressed in 1964. Resections are costly and recent reports have failed to show success. It is suggested that resections should be abandoned, but biologic research and palliation emphasized. INCIDENCE The incidence of pancreatic cancer has been increasing in western societies and is considered to become the second leading cause of cancer-related deaths by the year 2030 and a major burden. The incidence is estimated to be 64430 in the USA in 2021 and increase to 88,000 for both sexes in 2030. Incidence differs between ethnic groups for many cited between 10-15/100,000 but has been reported up to 20/100,000 in certain ethnic groups. RESERVATION, NON-RESECTED SURVIVORS  Resection for pancreatic adenocarcinoma was first attempted by Codivilla A in 1898 and then by Kausch W in 1912 [5] but was first successfully performed by Whipple A in 1935. Resection...

In a recent study, researchers revealed a draft genome sequence of the   monkeypox virus   associated with a recent outbreak in Portugal. Introduction Monkeypox is a rare disease caused by the monkeypox virus. It is a member of the same genus, including the variola virus causing smallpox, the Orthopoxvirus genus under the Poxviridae family. Monkeypox is a  zoonotic disease  endemic to tropical rainforest regions of West and Central Africa and is sometimes reported in other regions. The disease was first identified in a nine-year-old boy in the Democratic Republic of Congo in 1970. The enveloped monkeypox virus is a double-stranded (ds) deoxyribonucleic acid (DNA) virus with different rodents and non-human primates as hosts. The virus has been classified into two distinct genetic clades: the West African clade and the Central African (Congo Basin) clade. Symptoms are similar to those in  smallpox patients , albeit less severe. It spreads by direct contact with bo...

ETC-159 is a novel small molecule drug that targets a spread of cancers including colorectal, ovarian and pancreatic cancers. Its role is of an inhibitor in Wnt signalling pathways which promote cancer growth by allowing immune cells to access and enter the tumor. ETC-159 is been developed by partnership between [1]. ETC-159 inhibits an enzyme called porcupine, which prevents the secretion of Wnt proteins. ETC-159 is being tested in a clinical trial for use in cancers with overactive Wnt signalling, amongst other therapeutic indications during dose escalation in patients with advanced solid tumors; the utmost tolerated dose of ETC-159 is 30 mg every other day with dose-limiting toxicities and toxicity of concern of elevated serum b-CTX. ETC-159 with prophylactic denosumab is safe. ETC-159 has PD activity and increases immune infiltration. Currently ETC-159 dosing is ongoing at 24 mg. The signal used for DNA repair helps to stop mutations from developing in stem cells residing inside th...

Every Patient Involved in Drug Trial Found that Cancer vanishes Completely from their Body An Indian-origin patient was among those who had a " miracle " cure using a cancer medication that is being tried out by a research centre here, achieving an unprecedented healing rate when all 14 patients in the trial had their cancers disappear. "It's a miracle", Nisha Varughese said about the  immunotherapy  drug's efficacy in curing her. In the trial at the Memorial Sloan Kettering (MSK) Cancer Centre in New York using dostarlimab, "the rectal cancer disappeared after immunotherapy -- without the need for the standard treatments of radiation, surgery, or  chemotherapy  -- and the cancer has not returned in any of the patients, who have been cancer-free for up to two years," the MSK said. According to researchers, this was the first time that all the patients in a cancer trial were completely healed with a  medication  and they did not have serious side-ef...